vectrashield  (Vector Laboratories)

Journal: Molecules (Basel, Switzerland)

Article Title: Piezo1 Mechano-Activation Is Augmented by Resveratrol and Differs between Colorectal Cancer Cells of Primary and Metastatic Origin.

doi: 10.3390/molecules27175430

Figure Lengend Snippet: Figure 3. Constant shear stress decreases cell proliferation and increases CD133 expression in SW480 cells. (A) Percentage of proliferating cells 24 h post-FSS exposure, determined by the percentage of Ki67+ cells. N = 3, * p < 0.05 (one-way ANOVA with multiple comparisons). Error bars represent mean ± SD. (B) Cell-cycle distribution based on total DNA content from DAPI staining analyzed via

Article Snippet: Slides were assembled using 10 μL of Vectrashield antifade mounting media with DAPI (Vector Laboratories, Newark, CA, USA).

Techniques: Shear, Expressing, Staining

Journal: Molecules (Basel, Switzerland)

Article Title: Piezo1 Mechano-Activation Is Augmented by Resveratrol and Differs between Colorectal Cancer Cells of Primary and Metastatic Origin.

doi: 10.3390/molecules27175430

Figure Lengend Snippet: Figure 7. Resveratrol increases LR/Piezo1 colocalization, and SW480 cells have higher Piezo1 ex- pression. (A) Confocal microscopy images of Piezo1 (red), lipid rafts (green), DAPI (blue), and colocalization events between Piezo1 and LR (yellow). Scale bar = 30 µm. (B) Pearson’s corre- lation coefficient between LR/Piezo1 was calculated using the JACoP plugin in ImageJ. N = 3 (n = 15 images), * p < 0.05 (two-tailed unpaired t-test). For all plots, error bars represent mean ± SD. (C) Expression of Piezo1 (solid line) and isotype controls (dashed lines) in SW480 (red) and SW620 (blue) cells. (D) Median fluorescence intensity of Piezo1 normalized to the respective isotype control. N = 3, *** p < 0.001 (two-tailed unpaired t-test). (E) Protein abundance data (z-scores) from the Broad Cancer Cell Line Encyclopedia, downloaded from cBioPortal. Primary tumor and lymph node metastases from the same patient with colon adenocarcinoma (SW480 and SW620) and melanoma (WM115 and WM2664). Darker blue represents a higher z-score and higher Piezo1 abundance.

Article Snippet: Slides were assembled using 10 μL of Vectrashield antifade mounting media with DAPI (Vector Laboratories, Newark, CA, USA).

Techniques: Confocal Microscopy, Two Tailed Test, Expressing, Control, Quantitative Proteomics